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( A ) Schematic illustration of treatment regimen in mice. ( B ) The whole lungs were imaged and investigated after treatment by Masson staining. ( C ) The survival amount of each group on 30th day ( n = 6). ( D ) Quantification of fibrosis by Ashcroft score; each dot represents data from one animal; n = 6 biological independent animals. Ashcroft score was performed by averaging the score from two blinded and one nonblinded scorer. ( E ) Quantification of pulmonary hydroxyproline levels; n = 6 biological independent animals. ( F to H ) Histological examination of the nasal cavity (F), larynx (G), trachea, and lung (H) at 22th day. Alveoli of lungs are enlarged in the corresponding panels. Data are representative of two separate experiments each assayed in triplicate. Scale bars for the nasal cavity and larynx, 1000 μm. Scale bars for trachea and lung, 200 μm. ( I ) The levels of TGF-α, IFN-γ, IL-6, and IL-10 in serum and lung tissue measured by <t>ELISA</t> assay ( n = 6). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. Statistical significance was calculated via one-way ANOVA with a Tukey’s post hoc test.
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( A ) Schematic illustration of treatment regimen in mice. ( B ) The whole lungs were imaged and investigated after treatment by Masson staining. ( C ) The survival amount of each group on 30th day ( n = 6). ( D ) Quantification of fibrosis by Ashcroft score; each dot represents data from one animal; n = 6 biological independent animals. Ashcroft score was performed by averaging the score from two blinded and one nonblinded scorer. ( E ) Quantification of pulmonary hydroxyproline levels; n = 6 biological independent animals. ( F to H ) Histological examination of the nasal cavity (F), larynx (G), trachea, and lung (H) at 22th day. Alveoli of lungs are enlarged in the corresponding panels. Data are representative of two separate experiments each assayed in triplicate. Scale bars for the nasal cavity and larynx, 1000 μm. Scale bars for trachea and lung, 200 μm. ( I ) The levels of TGF-α, IFN-γ, IL-6, and IL-10 in serum and lung tissue measured by <t>ELISA</t> assay ( n = 6). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. Statistical significance was calculated via one-way ANOVA with a Tukey’s post hoc test.
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( A ) Schematic illustration of treatment regimen in mice. ( B ) The whole lungs were imaged and investigated after treatment by Masson staining. ( C ) The survival amount of each group on 30th day ( n = 6). ( D ) Quantification of fibrosis by Ashcroft score; each dot represents data from one animal; n = 6 biological independent animals. Ashcroft score was performed by averaging the score from two blinded and one nonblinded scorer. ( E ) Quantification of pulmonary hydroxyproline levels; n = 6 biological independent animals. ( F to H ) Histological examination of the nasal cavity (F), larynx (G), trachea, and lung (H) at 22th day. Alveoli of lungs are enlarged in the corresponding panels. Data are representative of two separate experiments each assayed in triplicate. Scale bars for the nasal cavity and larynx, 1000 μm. Scale bars for trachea and lung, 200 μm. ( I ) The levels of TGF-α, IFN-γ, IL-6, and IL-10 in serum and lung tissue measured by ELISA assay ( n = 6). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. Statistical significance was calculated via one-way ANOVA with a Tukey’s post hoc test.

Journal: Science Advances

Article Title: A softness zwitterionic micelles efficiently deliver inhaled nintedanib by enhancing airway mucus penetration

doi: 10.1126/sciadv.ady1030

Figure Lengend Snippet: ( A ) Schematic illustration of treatment regimen in mice. ( B ) The whole lungs were imaged and investigated after treatment by Masson staining. ( C ) The survival amount of each group on 30th day ( n = 6). ( D ) Quantification of fibrosis by Ashcroft score; each dot represents data from one animal; n = 6 biological independent animals. Ashcroft score was performed by averaging the score from two blinded and one nonblinded scorer. ( E ) Quantification of pulmonary hydroxyproline levels; n = 6 biological independent animals. ( F to H ) Histological examination of the nasal cavity (F), larynx (G), trachea, and lung (H) at 22th day. Alveoli of lungs are enlarged in the corresponding panels. Data are representative of two separate experiments each assayed in triplicate. Scale bars for the nasal cavity and larynx, 1000 μm. Scale bars for trachea and lung, 200 μm. ( I ) The levels of TGF-α, IFN-γ, IL-6, and IL-10 in serum and lung tissue measured by ELISA assay ( n = 6). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. Statistical significance was calculated via one-way ANOVA with a Tukey’s post hoc test.

Article Snippet: Mouse cytokine ELISA kits for IL-4 (catalog no. EK204/4-96), IL-10 (catalog no. EK210/4-96), TNF-α (catalog no. EK282/4-96) and TGF-β1 (catalog no. EK981-96), and IFN-γ (catalog no. EK280/3-96) were purchased from Multi Sciences (Lianke) Biotech Co. Ltd.

Techniques: Staining, Enzyme-linked Immunosorbent Assay

( A ) Schematic illustration of treatment regimen in mice. ( B ) The whole lungs were imaged and investigated after treatment by Masson staining. ( C ) The survival amount of each group on 30th day ( n = 6). ( D ) Quantification of fibrosis by Ashcroft score; each dot represents data from one animal; n = 6 biological independent animals. Ashcroft score was performed by averaging the score from two blinded and one nonblinded scorer. ( E ) Quantification of pulmonary hydroxyproline levels; n = 6 biological independent animals. ( F to H ) Histological examination of the nasal cavity (F), larynx (G), trachea, and lung (H) at 22th day. Alveoli of lungs are enlarged in the corresponding panels. Data are representative of two separate experiments each assayed in triplicate. Scale bars for the nasal cavity and larynx, 1000 μm. Scale bars for trachea and lung, 200 μm. ( I ) The levels of TGF-α, IFN-γ, IL-6, and IL-10 in serum and lung tissue measured by ELISA assay ( n = 6). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. Statistical significance was calculated via one-way ANOVA with a Tukey’s post hoc test.

Journal: Science Advances

Article Title: A softness zwitterionic micelles efficiently deliver inhaled nintedanib by enhancing airway mucus penetration

doi: 10.1126/sciadv.ady1030

Figure Lengend Snippet: ( A ) Schematic illustration of treatment regimen in mice. ( B ) The whole lungs were imaged and investigated after treatment by Masson staining. ( C ) The survival amount of each group on 30th day ( n = 6). ( D ) Quantification of fibrosis by Ashcroft score; each dot represents data from one animal; n = 6 biological independent animals. Ashcroft score was performed by averaging the score from two blinded and one nonblinded scorer. ( E ) Quantification of pulmonary hydroxyproline levels; n = 6 biological independent animals. ( F to H ) Histological examination of the nasal cavity (F), larynx (G), trachea, and lung (H) at 22th day. Alveoli of lungs are enlarged in the corresponding panels. Data are representative of two separate experiments each assayed in triplicate. Scale bars for the nasal cavity and larynx, 1000 μm. Scale bars for trachea and lung, 200 μm. ( I ) The levels of TGF-α, IFN-γ, IL-6, and IL-10 in serum and lung tissue measured by ELISA assay ( n = 6). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. Statistical significance was calculated via one-way ANOVA with a Tukey’s post hoc test.

Article Snippet: Mouse cytokine ELISA kits for IL-4 (catalog no. EK204/4-96), IL-10 (catalog no. EK210/4-96), TNF-α (catalog no. EK282/4-96) and TGF-β1 (catalog no. EK981-96), and IFN-γ (catalog no. EK280/3-96) were purchased from Multi Sciences (Lianke) Biotech Co. Ltd.

Techniques: Staining, Enzyme-linked Immunosorbent Assay